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Cloning and Expression
Enzymes
Bst2.0 WarmStartDNA Polymerase
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Bst2.0 WarmStartDNA Polymerase
Bst2.0 WarmStartDNA Polymerase from New England Biolabs
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Citations:
(36)
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New England Biolabs for
Bst2.0 WarmStartDNA Polymerase
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Company Info
Citations
Reviews
Product Specs
Item
Bst2.0 WarmStartDNA Polymerase
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0538M
Quantity
8000 units
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
DNA Polymerase
Temperature
-20 C
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (36)
(21)
Accessible LAMP-Enabled Rapid Test (ALERT) for Detecting SARS-CoV-2
Viruses
April 23, 2021
Ali Bektaş et al.
with 0.75 µL WarmStart Rtx (NEB, Ipswich, MA, USA, M0380) and 1.5 µL Bst 2.0 (
NEB
, Ipswich, MA, USA,
M0538L
). BPIFA1 RT-LAMP reactions were incubated at 63 °C for 30 min. A wax-layered cartridge that is ready
(22)
Contextual cues from cancer cells govern cancer-associated fibroblast heterogeneity
Cell reports
April 20, 2021
Bota-Rabassedas N, Banerjee P, Niu Y, Cao W, Luo J, Xi Y, Tan X, Sheng K, Ahn YH, Lee S,
et al
.
10X Thermopol Buffer, 0.5 μL 10 mM each dNTP, 0.3 μL Bst 2.0 WarmStart® DNA Polymerase (
NEB
, Cat. No.
M0538S
), and 6.6 μL PCR grade water to each sample. Twenty cycles of 60°C 20 s, 65°C 30 s were performed to
(23)
Genome-wide analysis of DNA replication and DNA double-strand breaks using TrAEL-seq
PLOS Biology
March 24, 2021
Neesha Kara, Felix Krueger, Peter Rugg-Gunn, Jonathan Houseley
isothermal amplification
buffer (NEB), 3 μl 100 mM MgSO4, 2 μl 10 mM dNTPs, and 1 μl Bst 2 WarmStart DNA polymerase (
NEB
M0538S
) was added and sample incubated 30 min at 65°C before precipitation with 12.5 μl 10 M NH4OAc, 1 μl GlycoBlue,
(24)
Genomic profiling of native R loops with a DNA-RNA hybrid recognition sensor
Science Advances
February 19, 2021
Kang Wang et al.
in 10 μl of 0.1%
Tween 20
. The eluent was mixed with 10 U of Bst 2.0 WarmStart DNA polymerase (
NEB
,
M0538
) in 1× Q5 polymerase reaction buffer and was incubated at 65°C for 30 min to perform the strand displacement
(25)
Genomes of Pleistocene Siberian Wolves Uncover Multiple Extinct Wolf Lineages
Current Biology
January 11, 2021
Jazmín Ramos-Madrigal et al.
T4 DNA Polymerase
,
New England Biolabs
Inc.,Cat#
M0203S
"BSt 2,0 warmstart polymerase",
New England Biolabs
Inc.,Cat#
M0538S
,, Deposited Data,Deposited Data,Deposited Data ,, "Tirekhtyakh (CGG32), Bunge-Toll-1885 (CGG29),
(26)
Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits
Scientific Reports
December 1, 2020
Erik Jue, Daan Witters, Rustem F. Ismagilov
µM Invitrogen bovine serum albumin (
15561020
,
Thermo Fisher Scientific
), 320 U/mL WarmStart Bst 2.0 (
M0538L
,
NEB
), and were supplemented with
nuclease-free water (not DEPC-Treated
,
4387936
,
Thermo Fisher Scientific
)
(27)
Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing
New England Journal of Medicine
October 8, 2020
Julia Joung et al.
Sigma M3409)
Tween 20
(
Millipore Sigma
P9416) Bst 2.0 WarmStart DNA Polymerase (
New England BioLabs
M0538L
)
WarmStart RTx Reverse Transcriptase
(
New England BioLabs
M0380L
) 100 mM Magnesium sulfate, supplied
(28)
RNA sequencing by direct tagmentation of RNA/DNA hybrids
Proceedings of the National Academy of Sciences
February 11, 2020
Lin Di et al.
RNA, the extension enzyme was replaced with 4 units of Bst 2.0 WarmStart DNA Polymerase (
NEB
, Cat. No.
M0538
). Correspondingly, the reaction temperature was up-regulated to 72 °C and inactivation was performed
(29)
Rapid and Sensitive Detection of Salmonella in Chickens Using Loop-Mediated Isothermal Amplification Combined with a Lateral Flow Dipstick
Journal of Microbiology and Biotechnology
March 28, 2019
Zhi Ke Liu, Qiu Yu Zhang, Ning-Ning Yang, Ming-Guo Xu, Jin-Feng Xu, Ming-Long Jing, Wen-Xing Wu, Ya-Dong Lu, Feng Shi, Chuang-Fu Chen
and dNTP mix from Sangon Biotech Co., Ltd. (China). We bought Bst 2.0 DNA polymerase large fragment (
M0538
) from
New England Biolabs
, Ltd. (China). We obtained buffered peptone water (BPW) and
Rappaport
Vassiliadis
(30)
Rapid Multiplex Small DNA Sequencing on the MinION Nanopore Sequencing Platform
G3 Genes|Genomes|Genetics
May 1, 2018
Shan Wei, Zachary R. Weiss, Zev Williams
reaction mixture with addition of 0.9 µL 100 mM dATP and 1µL Bst 2.0 WarmStart DNA polymerase (
NEB
, Cat.
M0538S
) before the 65°
incubation
. Each reaction mixture was then purified using a DNA Clean & Concentrator-5
(31)
SiC-Seq: Single-cell genome sequencing at ultra high-throughput with microfluidic droplet barcoding
Nature Biotechnology
July 1, 2017
Freeman Lan, Benjamin Demaree, Noorsher Ahmed, Adam R Abate
FC-131-1024), 1% (w/v) Tween 20, 1% (w/v) PEG-6000, 2.5 U/μL Bst 2.0 WarmStart DNA Polymerase (
NEB
, catalog no.
M0538S
) are each loaded into a 1 mL syringe and injected into the sequential merger device as shown in Supplementary
(32)
Droplet barcoding for massively parallel single-molecule deep sequencing
Nature Communications
September 1, 2016
Freeman Lan, John R. Haliburton, Aaron Yuan, Adam R. Abate
ligation
mix of: 1 μM adaptors, 0.20 mM dNTPs, 0.5 μl (60 units) of Bst 2.0 polymerase warmstart (
NEB
:
M0538M
), 2.5 μl
T4 DNA ligase
from the quick
ligation
kit (
NEB
:
M2200S
), 1 × ligase buffer from the quick ligase
(33)
Surveillance for Western Equine Encephalitis, St. Louis Encephalitis, and West Nile Viruses Using Reverse Transcription Loop-Mediated Isothermal Amplification
PLOS ONE
January 25, 2016
Sarah S. Wheeler, Cameron S. Ball, Stanley A. Langevin, Ying Fang, Lark L. Coffey, Robert J. Meagher
(Sigma #B-0300), 1.4 mM each dNTP (
NEB
#
N0447L
), 0.32 units/μL Bst 2.0 WarmStart DNA polymerase (
NEB
#
M0538M
), 0.2 units/μL
AMV reverse transcriptase
(
NEB
#
M0277T
, or Life Science Advanced Technologies #AMVRTT-5),
(34)
NGS-based deep bisulfite sequencing
MethodsX
January 1, 2016
Suman Lee, Joomyeong Kim
WarmStart DNA polymerase (includes 10×
Isothermal Amplification
Buffer;
New England Biolabs
. Cat. No.
M0538S
) "DNA Clean & Concentrator™-25 (includes spin columns, DNA Binding Buffer, Wash Buffer, Elution Buffer,
(35)
SELMA: Selection with Modified Aptamers
Current Protocols in Chemical Biology
November 1, 2015
J. Sebastian Temme, Isaac J. Krauss
BST 2.0 WarmStart DNA polymerase with 10× ThermoPol reaction buffer (
New England Biolabs
, cat. no.
M0538
) 10 μM forward primer: 5′ -TGCGGTTATGAGGTGGAGTT-3′ 10 mM dNTP mix (see recipe) Binding buffer + 0.02%
(36)
Direct Blood Dry LAMP: A Rapid, Stable, and Easy Diagnostic Tool for Human African Trypanosomiasis
PLOS Neglected Tropical Diseases
March 13, 2015
Kyoko Hayashida, Kiichi Kajino, Lottie Hachaambwa, Boniface Namangala, Chihiro Sugimoto
Bst DNA polymerases; Bst 2.0 WarmStart DNA Polymerase 8,000 U/ml (
NEB
M0538L
) and 120,000 U/ml (
NEB
M0538M
), so that proper drying time and
enzyme stability
were achieved. The primer/CFI solution and enzyme
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Bst2.0 WarmStartDNA Polymerase from New England Biolabs
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